Cancer Cell Culture

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April 2011



With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success - only by good practice and quality control will the results of cancer cell culture improve.  Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.


1. Cancer Biology
            Ian A. Cree
2. Principles of Cancer Cell Culture
            Ian A. Cree
3. Storage of Cell Lines
            Katharine A. Parker
4. Characterization and Authentication of Cancer Cell Lines: An Overview
            Yvonne A. Reid
5. Online Verification of Human Cell Line Identity by STR DNA Typing
            Wilhelm G. Dirks and Hans G. Drexler
6. Cytogenetic Analysis of Cancer Cell Lines
            Roderick A. F. MacLeod and Hans G. Drexler
7. Cell Culture Contamination
            Glyn N. Stacey
8. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
            Cord C. Uphoff and Hans G. Drexler
9. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
            Cord C. Uphoff and Hans G. Drexler
10. Quality Assurance and Good Laboratory Practice
            Louise A. Knight and Ian A. Cree
11. Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning
            Laura Breen, Joanne Keenan, and Martin Clynes
12. Isolation and Culture of Colon Cancer Cells and Cell Lines
            Sharon Glaysher and Ian A. Cree
13. Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
            Julia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett
14. Isolation and Culture of Squamous Cell Carcinomalines
            Karin J. Purdie, Celine Pourreyron, and Andrew P. South
15. Isolation and Culture of Ovarian Cancer Cells and Cell Lines
            Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, Jutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz
16. Establishment and Culture of Leukemia-Lymphoma Cell Lines
            Hans G. Drexler
17. Isolation of Inflammatory Cells from Human Tumors
            Marta Polak
18. Isolation of Endothelial Cells from Human Tumors
            Elisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, Roland S. Croner, and Michael Stürzl
19. Cellular Chemosensitivity Assays: An Overview
            Venil N. Sumantran
20. Cell Sensitivity Assays: The MTT Assay
            J. van Meerlo, G.J.L. Kaspers, and J.Cloos
21. Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay
            Sharon Glaysher and Ian A. Cree
22. The Differential Staining Cytotoxicity (DiSC) Assay: A Review
            Larry M. Weisenthal
23. Real-time Cytotoxicity Assays
            Donald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, and Jonathan M. Cooper
24. FPurification of Annexin V and its use in the Detection of Apoptotic Cells
            Katy M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss
25. Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay
            Janet M. Hartley, Victoria J. Spanswick, and John A. Hartley
26. Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR
            Björn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod
27. Cell Migration and Invasion Assays
            K.A. Moutasim, M.L. Nystrom, and G.J. Thomas
28. Angiogenesis Assays
            V. Poulaki
29. Flow Cytometric DNA Analysis of Human Cancers and Cell Lines
            Sarah A. Krueger and George D. Wilson
30. Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines
            Stefan Nagel and Hans G. Drexler
31. Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transciptase Polymerase Chain Reaction
            Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree
32. Proteomic Evaluation of Cancer Cells - Identification of Cell Surface Proteins
            Samantha Larkin and Claire Hastie
33. Development of Rituximab-resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-mediated Immunotherapy
            Ali R. Jazirehi and Bejamin Bonavida
34. High Throughput Screening of Natural Products for Cancer Therapy: A Review
            Alan L. Harvey and Ian A. Cree
35. Analysis of Drug Interactions
            Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters
36. Transfection and DNA-mediated Gene Transfer
            Davide Zecchin and Federica Di Nicolantonio
37. Drug Design and Testing: Profiling of Anti-proliferative Agents for Cancer Therapy Using a Cell-based Methyl-[3H]-Thymidine Incorporation Assay
            Matthew Griffiths and Hardy Sundaram
38. Feeder Layers: Co-culture with Non-neoplastic Cells
            Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South
39. Xenotransplantation of Breast Cancers
            Massimiliani Cariati, Rebecca Marlow, and Gabriela Dontu

EAN: 9781617790799
ISBN: 1617790796
Untertitel: Methods and Protocols. 2nd ed. 2011. Book. Sprache: Englisch.
Verlag: Humana Press
Erscheinungsdatum: April 2011
Seitenanzahl: 516 Seiten
Format: gebunden
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