EBOOK

Evolutionary Methods in Biotechnology


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Mai 2004

Beschreibung

Beschreibung

Wie erstellt und durchsucht man kombinatorische Enzymbibliotheken? Dieser Band stellt Ihnen erstmals geprüfte, ausführliche Protokolle zur Verfügung. Die Autoren der Beiträge, Experten mit eigener Erfahrung in der evolutionären Biotechnologie, kommentieren die Arbeitsanleitungen mit Hinweisen zu häufigen Fehlern und deren Beseitigung. Unentbehrlich für jeden, der auf diesem interdisziplinären Gebiet arbeitet.

Inhaltsverzeichnis

Introduction
Generation of Mutant Libraries Using Random Mutagenesis
DNA Shuffling
DNA Recombination Using StEP
FACS Screening of Combinatorial Peptide and Protein Libraries
Selection of Phage-displayed Enzymes
Selection of Aptamers
Methods for Selecting Catalytic Nucleic Acids
High-Throughput Screening of Enantioselective Industrial Biocatalysts
Computer-assisted Design of Doped Libraries
Directed in silico Mutagenesis
RNA Folding in silico
Patenting in Evolutionary Biotechnology
Programs on CD: How to identify positions suited for mutagenesis; Folding aspects; databases; structure-based design; and more
Glossary and Index

Portrait

Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received her diploma in 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Göttingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications.

Andreas Schwienhorst is a group leader at the Institute of Microbiology and Genetics at the University of Göttingen (Germany). He was born in Warendorf and did his studies of Biochemistry and Prehistory in Münster and Göttingen. His thesis was carried out at the Max-Planck-Institute for Biophysical Chemistry under the supervision of Manfred Eigen. He was a visiting scientist at the Salk Institute, La Jolla and the Los Alamos National Laboratory (USA), then took a position as a group leader at the Institute for Molecular Biotechnology before moving to the University of Göttingen in 1997. In 1998 he received the Biofuture Award. Currently, he is interested in the discovery of novel targets for drug intervention as well as in methods of molecular evolution and their applications in drug discovery and catalysis.



EAN: 9783527307999
ISBN: 3527307990
Untertitel: Dirty Tricks for Directed Evolution. New. 115 schwarz-weiße Abbildungen, 11 schwarz-weiße Tabellen. Book with CD-ROM. Inklusive CD-ROM.
Verlag: Wiley VCH Verlag GmbH
Erscheinungsdatum: Mai 2004
Seitenanzahl: 250 Seiten
Format: gebunden
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