EBOOK

Clinical and Forensic Applications of Capillary Electrophoresis


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April 2001

Beschreibung

Beschreibung

Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Inhaltsverzeichnis

Section I. Introduction

Capillary Electrophoresis: General Overview and Applications in the Clinical Laboratory
Manjiri Lele, Subodh M. Lele, John R. Petersen, and Amin A. Mohammad

Basic Principles and Modes of Capillary Electrophoresis
Harry Whatley

Section II. Protein Electrophoresis

Capillary Coatings for Protein Analysis
Kannan Srinivasan, Chris Pohl, and Nebojsa Avdalovic

Clinical Serum Protein Capillary Zone Electrophoresis
Carl R. Jolliff

Serum and Urine Paraprotein Capillary Electrophoresis
Carl R. Jolliff

Cerebrospinal Fluid Protein Electrophoresis
John R. Petersen and Amin A. Mohammad

Lipoprotein Analysis
Rainer Lehmann

Clinical Analysis of Structural Hemoglobin Variants and Hb A1c by Capillary Isoelectric Focusing
James M. Hempe, Alfonso Vargas, and Randall D. Craver

Section III. Metabolic Diseases

Amino Acid Analysis, Mary Kathryn Linde. Organic Acids
Kern L. Nuttall and Norberto A. Guzman

Steroids, Cecilla Youh
Amin A. Mohammad, and John R. Petersen

Section IV. Immunoassay

Capillary Electrophoresis Based Immunoassay
Bode Adesoji, Amin A. Mohammad, and John R. Petersen

Section V. Molecular Diagnostics

Quantitation of Viral Load
Jill M. Kolesar

The Application of Capillary Electrophoresis in the Analysis of PCR Products used in Forensic DNA Typing
Bruce R. McCord and John M. Butler

Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry
Samir Cherkaoui

Section VI. Mass Spectrometry, Therapeutic Drug Monitoring, and Toxicology

Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins
Stephen Naylor and Andy J. Tomlinson

Serum Drug Monitoring by Capillary Electrophoresis
Zack K. Shihabi

Applications of Capillary Zone Electrophoresis in the Analysis of Metal Ions of Clinical Significance
Lokinendi V. Rao, John R. Petersen, Amin A. Mohammad, and Anthony O. Okorodudu

Clinical and Forensic Drug Toxicology: Analysis of Illicit and Abused Drugs in Urine by Capillary Electrophoresis
Wolfgang Thormann and Jitka Caslavska

Screening Biological Specimens for Drugs of Forensic Significance
John C. Hudson, Murray J. Malcolm, and Mauro Golin

Pressestimmen

"This book fills a niche for those curious about this technology. It would be of greatest use to those with an academic interest in capillary electrophoresis."-Doody's Health Sciences Book Review Journal
EAN: 9780896036451
ISBN: 0896036456
Untertitel: 2001. Auflage. Sprache: Englisch.
Verlag: SPRINGER VERLAG GMBH
Erscheinungsdatum: April 2001
Seitenanzahl: 454 Seiten
Format: gebunden
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