RNA Interference Technology: From Basic Science to Drug Development

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Dezember 2004



2005 overview of RNA interference (RNAi) technology, covering both fundamental science and applications.


Part I. Basic RNAi, siRNA and Gene Silencing Mechanisms: 1. RNAi beginnings: overview of the pathway in C. elegans; 2. Dicer in RNAi: its roles in vivo and utility in vitro; 3. Genes required for RNAi; 4. MicroRNAs: a small contribution from worms; 5. miRNAs in the brain and the application of RNAi to neurons; Part II. Design and Synthesis of siRNAs: 6. Design and synthesis of small interfering RNA (siRNA); 7. Automated design and high throughput synthesis of siRNA; 8. Rational design of siRNAs with the Sfold software; 9. Enzymatic production of siRNAS; Part III. Vector Development and In Vivo, In Vitro and In Ovo Delivery Methods: 10. Six methods of inducing RNAi in mammalian cells; 11. Viral delivery of siRNA; 12. siRNA delivery by lentiviral vectors: design and applications; 13. Liposomal delivery of siRNAs in mice; 14. Chemical modifications to achieve increased stability and sensitive detection of siRNA; 15. RNAi in postimplantation mouse embryos; 16. In ovo RNAi opens new possibilities for functional genomics in vertebrates; Part IV. Gene Silencing in Model Organisms: 17. Practical applications of RNAi in C. elegans; 18. Inducible RNAi as a forward genetic tool in Trypanosoma brucei; 19. RNAi gene silencing in yeast; 20. RNA silencing in filamentous fungi: Mucor circinelloides as a model organism; 21. RNAi and gene silencing phenomenon mediated by viral suppressors in plants; Part V. Drug Target Validation: 22. Delivering siRNA in vivo for functional genomics and novel therapeutics; 23. The role of RNAi in drug target validation: application to Hepatitis C; 24. RNAi in the drug discovery process; 25. RNAi: use in the drug discovery process; Part VI. Therapeutic and Drug Development: 26. RNAi-mediated silencing of viral gene expression and replication; 27. RNAi in drug development: practical considerations; 28. RNAi for the treatment of liver diseases; 29. RNAi applications in animal systems; Part VII. Genome Wide RNAi and High Through-Put Analysis: 30. High through-put RNAi-by-soaking in Caenorhabditis elegans; 31. Tools for integrative genomics: genome-Wide RNAi and expression profiling in Drosophila; 32. Microarray analysis and RNA silencing to determine genes functionally important in Mesothelioma; 33. High through-put RNAi; 34. Generation of highly specific vector-based shRNAi libraries directed against the entire human genome.


Krishnarao Appasani is the Founder of GeneExpression Systems, a gene discovery company focusing on functional genomics in cancer research.


Review of the hardback: 'This book is very timely. The chapters on the basics of RNAi, siRNA and miRNAs are an excellent summary of the recent origins of this topic and the various sections dealing with RNAi technology provided comprehensive background material. I am sure that, even with the rapid progress in RNAi , this book will be useful for both newcomers to the field wishing to get an overview and for experienced researchers looking for a summary of published information.' David Baulcombe The Sainsbury Laboratory, Norwich UK Review of the hardback: 'Only six years ago Fire and Mello discovered RNAi, as a funny effect in little worms. Now the whole world wants to know about it. In this book Krishnarao Appasani got an all-star crowd of specialists to introduce RNAi: its history, biology, application in genomics research, and possible therapeutic use. Warmly recommended.' Ronald Plasterk Hubrecht Laboratories, Utrecht, the Netherlands Review of the hardback: 'A comprehensive treatise on RNA interference has long been needed. That need has now been filled with this book, which places RNAi and its potential squarely in the context of the post human genome era. The successful juxtaposition of the many aspects of RNAi biology and technology should serve to stimulate creative thought across the many diverse disciplines affected by the RNAi revolution.' Ronald K. Scheule Ph.D., Genzyme Corporation Review of the hardback: 'This new book is very comprehensive, in many ways picking up where Hannon's RNAi manual leaves off. A good companion to Hannon.' Frank Slack Ph.D. Yale University Review of the hardback: 'This extensive collection of timely articles brings us up to date on the current research on RNAi, its science and applications. It is a must read to learn about current status of the art. Its 35 contributions from leading scientists are subdivided into seven sections to facilitate access to the corresponding articles, and cover every aspect of the subject. This is a rapidly advancing field and it is useful for the newcomer both in academia and in industry to obtain a panoramic view and put the current literature in perspective.' Roberto Weinmann Bristol-Myers Squibb Review of the hardback: 'This book provides a window to the exciting new RNA interference revolution with special emphasis on applications for biotechnology. It will provide up-to-date background for academic and industry researchers seeking to make use of this powerful research tool.' Judy Leiberman Harvard Medical School and The CBR Institute for Biomedical Research Review of the hardback: '... this most impressive book is not only scientifically informative but also visually appealing. I would have no hesitation recommending this book to anyone interested in RNAi research, particularly newcomers to this daunting area. I am sure this book will become a standard reference point for laboratories working in this area.' Biologist Review of the hardback: 'This book provides an excellent, comprehensive description of the current state of the art, in a clear and readily absorbable format, suitable for a wide scientific audience.' Society for General Microbiology
EAN: 9780521836777
ISBN: 0521836778
Untertitel: New. Sprache: Englisch.
Erscheinungsdatum: Dezember 2004
Seitenanzahl: 544 Seiten
Format: gebunden
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