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Cell Migration in Inflammation and Immunity: Methods and Protocols


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August 2003

Beschreibung

Beschreibung

Chemokines and their receptors play a central role in the pathogenesis of numerous, perhaps all, acute and chronic inflammatory diseases. About 50 distinct chemokines produced by a variety cell types and tissues either c- stitutively or in response to inflammatory stimuli are involved in a plethora of biological processes. These small secreted proteins exert their exquisitely variegated functions upon binding to a family of seven-transmembrane spanning G-protein coupled receptors (GPCRs) composed of almost 20 distinct entities. The biological activities of chemokines range from the control of leukocyte trafficking in basal and inflammatory conditions to the regulation of hema- poiesis, angiogenesis, tissue architecture, and organogenesis. The basis for such diversified activities rests, on one hand, upon the ubiquitous nature of chemokine production and chemokine receptor expression. Virtually every cell type can produce chemokines and expresses a unique combination of chemokine receptors. On the other hand, chemokine receptors make use of a flexible and complex network of intracellular signaling machineries that can regulate a variety of cellular functions ranging from cell migration, growth, and differentiation to death. As knowledge of the size of chemokine and chemokine receptor families rapidly reaches completeness, much is still to be uncovered in terms of fu- tional architecture of the chemokine system. The disparity between the large number of chemokines and that smaller number of receptors is balanced by the promiscuity in ligand-receptor interactions, with multiple chemokines binding to the same receptor and several chemokines binding to more than one receptor.

Inhaltsverzeichnis

Chemotaxis and Interaction with Vascular or Lymphatic Endothelium
Silvano Sozzani, Annunciata Vecchi, Paola Allavena, and Alberto Mantovani

Analysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow Conditions
Carlo Laudanna

Posttranslational Processing of Chemokines
Paul Proost, Frank Mahieu, Evemie Schutyser, and Jo Van Damme

Chemotactic Profiling of Lymphocyte Subpopulations
Lucia Colantonio, Andrea Iellem, and Daniele D'Ambrosio

Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow Cytometry
Miguel Vicente-Manzanares, Mariano Vitón, and Francisco Sánchez-Madrid

Evaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation on Human Leukocytes
Angela Gismondi, Fabrizio Mainiero, and Angela Santoni

Reconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted Tracking
Peter Friedl and Eva-B. Bröcker

Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System
Mark A. Landree and Peter N. Devreotes

Conditional Transgenic Models to Study Chemokine Biology
Sergio A. Lira, Borna Mehrad, Shu-Cheng Chen, Petronio Zalamea, David J. Kinsley, Maria T. Wiekowski, Elizabeth Coronel, Galya Vassileva, Denise Manfra, and Kristian K. Jensen

Intravital Microscopy as a Tool for Studying Recruitment and Chemotaxis
Denise C. Cara and Paul Kubes

Tracking Antigen-Specific Lymphocytes In Vivo
Claire L. Adams, Catherine M. Rush, Karen M. Smith, and Paul Garside

Analysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease States
Margherita Mariani and Paola Panina-Bordignon

Interaction of Viral Chemokine Inhibitors with Chemokines
Antonio Alcami

Discovery of Small-Molecule Antagonists ofChemokine Receptors: Screening Strategy and Assays
Maria Elena Fuentes

Visualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital Microscopy
Gabriela Constantin

Animal Models to Study Chemokine Receptor Function In Vivo: Mouse Models of Allergic Airway Inflammation
Clare M. Lloyd and Jose-Carlos Gutierrez-Ramos

Assessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling: Use of Dominant Negative Mutants Controlled by a Tetracycline-Regulated Gene Expression System
Adam P. Curnock, Yannis Sotsios, and Stephen G. Ward

In Vitro and In Vivo Models to Study Chemokine Regulation of Angiogenesis
Giovanni Bernardini, Domenico Ribatti, Gaia Spinetti, Lucia Morbidelli, Marina Ziche, Angela Santoni, Maurizio C. Capogrossi, and Monica Napolitano

Real Time In Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow Conditions
Guy Cinamon and Ronen Alon

Generation of Monoclonal Antibodies Against Chemokine Receptors
Leonor Kremer and Gabriel Márquez

Detection of High-Affinity a4-Integrin Upon Leukocyte Stimulation by Chemoattractants or Chemokines
Jason R. Chan and Myron I. Cybulsky

Index
EAN: 9781588291028
ISBN: 1588291022
Untertitel: 'Methods in Molecular Biology'. 2003. Auflage. Sprache: Englisch.
Verlag: SPRINGER VERLAG GMBH
Erscheinungsdatum: August 2003
Seitenanzahl: 277 Seiten
Format: gebunden
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