Interleukin Protocols

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Juli 2001



Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clear upon analysis of the human genome sequence. Most important, interleukins are central to the pathogenesis of a wide range of diseases that involve an immune com- nent, including such conditions as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, psoriasis, and asthma. Interleukins have also been imp- cated in other conditions, including cancer, migraine, myocardial infarction, and depression. In essence, when cells are activated by interleukins, a program of gene expression is initiated in the target cell that alters the cell's phenotype, leading to enhanced immune reactivity, inflammation, and/or proliferation. Interleukins are therefore at the core of the cellular basis for many diseases. They are the subject of intense investigation by biomedical researchers and the targeting or use of interleukins in the clinic is proceeding apace. Approaches such as t- geting IL-4 in asthma or IL-1 in joint disease are being pursued, and it is likely that in the next 5-10 years a number of new therapies based on either inhib- ing or administering interleukins will be available.


Part I: General Methods for Assaying Interleukin Protein and mRNA

ELISAs and Interleukin Research
Catherine Greene

ELISPOT Technique for Assaying Interleukins
Almuth Christine Hauer and Mona Bajaj-Elliott

Measurement of Interleukins by ELISPOT Assay with Particular Application for Dual Color Analysis (Stardust Assay)
Yoshihiro Okamato and Mikio Nishida

Assaying Interleukins Intracellularly by FACS
Liam O'Mahony and Maurice O'Donoghue

Basic RT-PCR for Measurement of Cytokine Expression
Mona Bajaj-Elliot and Almuth Christine Hauer

Quantitative PCR for Measurement of Cytokine Expression
Ellen M. Nilsen, Finn-Eirik Johnasen, and Per Brandtzaeg

Single Cell RT-PCR Method for the Detection of Cytokine mRNA
Moshe Ligumsky, Hovav Nechushtan, and Ehud Razin.

uantitation of Cytokine mRNA by Flash-Type Bioluminescence
Jeffrey K. Actor

Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method
Ginette Tardif, John A. Di Battista, Jean-Pierre Pelletier, and Johanne Martel-Pelletier

Quantification of Mouse IL-6 and TNF-_ mRNA Using Xplore Assays
Scott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson and Mark D. Moody

Measurement of Cytokine and Chemokine mRNA Using Nonisotopic MultiProbe RNase Protection Assay
Zheng-Ming Wang and Roman Dziarski

Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography
Terry M. Phillips and Benjamin F. Dickens

Detection of Cytokines by Immunohistochemistry
Mahmoud Huleihel

Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement
Nicolai Petrovsky

Part II: Illustration of Methodologies Using Individual Interleukins

Assaying Interleukin-8
Paul N. Moynagh

Measurement of Interleukin-2 by Bioassay
Lyanne Weston

Assaying Interleukin-15
Amos Douvdevani, Michal Weiler, and Eli Lewis

Part III: Assaying Interleukins in Particular Pathologies

Assaying Interleukin-6 in Breast Cancer
Judith Harmey, Amanda Haverty, Deirdre Foley and David Bouchier-Hayes

Cytokines, Stress, and Depressive Illness
Hymie Anisman, Shawn Hayley, Arun V. Ravindran, Zul Merali, and Jenna Griffiths

Measurement of Interleukins in Cutaneous Disorders
Khusru Asadullah, Antje Haeussler-Quade, Wolf Dietrich Doecke, Wolfram Sterry

Interleukins in Graves' Disease
Jan Komorowski

Evaluation of Cytokines in Migraine Patients by ELISA
Irene Munno, Mariarosaria Marinaro, Giuseppe Lacedra, Antonia Bassi, and Vincenzo Centonze

Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction
Radek Pudil

Part IV: Assaying Interleukins in Particular Biological Fluids

Measurement of Cytokines in Peritoneal Fluids
Reijo Punnonen and Juha Punnonen

Measurement of Cytokines in Seminal Plasma
Marek Glezerman, Eitan Lunenfeld, and Mahmoud Huleihel

Measurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPD
Peter G. Gibson and Jodie L. Simpson

Cytokines in Blister Fluid
Franco Ameglio and Luciano D'Auria

Measurement of Cytokines in Synovial Fluid
Fabrizio De Benedetti

Measurement of Cytokines in Cerebrospinal Fluid
Toshiyuki Yokoyama

Detection of Interleukins in Tears
Archana Thakur and Mark D. P. Willcox

Part V: Newer Techniques: Interleukin Signal Transduction, Recombinant Interleukin Production, Interleukin Gene Polymorphisms, and the use of cDNA Arrays

Measurement of Phosphoinositide 3-Kinase Activation by Interleukins
Stephen G. Ward

Analysis of Interleukin-2 S


"this volume offers protocols for measurement of various interleukins. Most are of sufficient detail so as to serve as a laboratory guide for investigators. Investigators with a specific interest in analyzing a given interleukin will find this volume helpful. "-Doody's Health Sciences Book Review Journal
"Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics." - Journal of Investigational Allergology and Clinical immunology
"A variety of techniques is represented in 36 chapters which are written in a concise, uniform format b y authors who obviously have a lot of hands-on experience with the methods covered...If you are working in this area, put this book on a shelf in the lab and it will not be just recently started graduate students who will gracefully turn to it. - Chembiochem
"The protocols that are described in the book are user friendly, in the sense that they are not only well detailed and easy to follow but also reliable. The researchers that are naive in the field will find a lot of useful information in the book whereas the experienced ones will be able to use the book as a source of additional information on the methods already in use. A must therefore for the psychoneuroendocrinologist with a working interest in the relationships between the (neuroendocrine) brain and the immune system." -Psychoneuroendocriniology
EAN: 9780896037380
ISBN: 089603738X
Untertitel: 'Methods in Molecular Medicine'. 2001. Auflage. Book. Sprache: Englisch.
Verlag: Humana Press
Erscheinungsdatum: Juli 2001
Seitenanzahl: 464 Seiten
Format: gebunden
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