Malaria Methods and Protocols

€ 241,99
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Juni 2002



The Plasmodium spp. parasite was identified as the causative agent of malaria in 1880, and the mosquito was identified as the vector in 1897. Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria. Physical barriers (bed nets, window screens) and chemical prevention methods (insecticides and mosquito repellents) intended to interfere with the transmission of the disease are not highly effective, and the profile of resistance of the parasite to chemoprophylactic and chemotherapeutic agents is increasing. The dawn of the new millennium has seen a resurgence of interest in the disease by government and philanthropic organizations, but we are still faced with compl- ities of the parasite, the host, and the vector, and the interactions among them. Malaria Methods and Protocols offers a comprehensive collection of protocols describing conventional and state-of-the-art techniques for the study of malaria, as well as associated theory and potential problems, written by experts in the field. The major themes reflected here include assessing the risk of infection and severity of disease, laboratory models, diagnosis and typing, molecular biology techniques, immunological techniques, cell biology techniques, and field applications.


Part I. Assessing Risk of Infection and Severity of Disease

Vector Incrimination and Entomological Inoculation Rates
John C. Beier

Epidemiological Measures of Risk of Malaria
J. Kevin Baird, Michael J. Bangs, Jason D. Maguire, and Mazie J. Barcus

Part II. Laboratory Models

Maintenance of the Plasmodium berghei Life Cycle
Robert E. Sinden, Geoff A. Butcher, and A. L. Beetsma

Mouse Models for Pre-Erythrocytic-Stage Malaria
Laurent Rénia, Elodie Belnoue, and Irène Landau

Mouse Models for Erythrocytic-Stage Malaria
Latifu A. Sanni, Luis F. Fonseca, and Jean Langhorne

Nonhuman Primate Models : I. Nonhuman Primate Host-Parasite Combinations
William E. Collins

Nonhuman Primate Models : II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivax
William E. Collins

Part III. Diagnosis and Typing

Vector Analysis
John C. Beier

Genotyping of Plasmodium spp.: Nested PCR
Georges Snounou

Genotyping of Plasmodium falciparum: PCR-RFLP Analysis
Ingrid Felger and Hans-Peter Beck

Microsatellite Analysis in Plasmodium falciparum
Xin-zhuan Su and Michael T. Ferdig

Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR
Adam A. Witney, Robert M. Anthony, and Daniel J. Carucci

Quantitation of Liver-Stage Parasites by Competitive RT-PCR
Kyle C. McKenna and Marcelo R. S. Briones

Part IV. Molecular Biology Techniques

Extraction and Purification of Plasmodium Total RNA
Till Voss

Extraction and Purification of Plasmodium Parasite DNA
Hans-Peter Beck

Southern Blotting of Parasite DNA
Tobias Spielmann

SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins
Roland A. Cooper

Nested PCR Analysis of Plasmodium Parasites
Georges Snounou and BalbirSingh

RFLP Analysis
Hans-Peter Beck

Analysis of Gene Expression by RT-PCR
Peter Preiser

In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites
Joanne Thompson

Purification of Chromosomes from Plasmodium falciparum
Daniel J. Carucci, Paul Horrocks, and Malcolm J. Gardner

Construction of Genomic Libraries from the DNA of Plasmodium Species
Leda M. Cummings, Dharmendar Rathore, and Thomas F. McCutchan

Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA
Dharmendar Rathore and Thomas F. McCutchan

Construction of Plasmodium falciparum l cDNA Libraries
David A. Fidock, Dharmendar Rathore, and Thomas F. McCutchan

Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization
David A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen, and Anthony A. James

Construction and Screening of YAC Libraries
Cecilia P. Sanchez, Martin Preuss, and Michael Lanzer

Episomal Transformation of Plasmodium berghei
Chris J. Janse and Andrew P. Waters

Gene Targeting in Plasmodium berghei
Vandana Thathy and Robert Ménard

Part V. Immunological Techniques

Peptide Vaccination, Valentin Meraldi
Jackeline F. Romero, and Giampietro Corradin

DNA Vaccination
Richard C. Hedstrom and Denise L. Doolan

Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses
Moriya Tsuji

Assessing CD4+ Helper T-Lymphocyte Responses by Lymphoproliferation
Isabella A. Quakyi and Jeffrey D. Ahlers

Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice
Elsa Seixas, Jean Langhorne, and Stuart Quin

Cell Trafficking: Malaria Blood-Stage Parasite-Specific CD4+ T Cells After Adoptive Transfer into Mice


"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Laboratory International
"This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters." -Molecular Biotechnology
EAN: 9780896038233
ISBN: 0896038238
Untertitel: 'Methods in Molecular Medicine'. 2002. Auflage. Sprache: Englisch.
Erscheinungsdatum: Juni 2002
Seitenanzahl: 631 Seiten
Format: gebunden
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