Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols

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Juli 2002



In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with "oxidative stress" using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Western blotting are the most widely used. Several forms of therapy are now available for patients with increased oxidative stress. In addition to standard antioxidant therapy supplementation in vivo and in vitro, photodynamic therapy (PDT) employs excitation of a photon-emitting compound delivered systemically for free radical-mediated necrosis of affected tissues, and stem cells are also being used to induce signaling events or replace antioxidant enzymes.


Part I. Techniques for Histological Identification

Cytochemical Localization of H2O2 in Biological Tissues
E. Ann Ellis and Maria B. Grant

Localization of Intracellular Lipid Peroxides Using Tetramethylbenzidine Reaction for Transmission Electron Microscopy
E. Ann Ellis, Shigehiro Iwabuchi, Don Samuelson, and Donald Armstrong

The Immunohistochemical Localization of Glutathione Peroxidase
Kiyoshi Akeo, Tadahisa Hiramitsu, and Keiichi Watanabe

4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
Hideyuki J. Majima, Takako Nakanishi-Ueda, and Toshihiko Ozawa

Immunohistochemical Detection of Protein Oxidation
Jürgen Frank, Alfonso Pompella, and Hans K. Biesalski

Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells
Alfonso Pompella, Silvia Dominici, Jürgen Frank, and Hans K. Biesalski

Part II. Techniques for Subcellular Localization

Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts
(AGE Receptors)
Paul J. Thornalley

Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes
Kiyomi Kikugawa

X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress
R. Preston Mason and Robert F. Jacob

Detection of Estrogen Receptor by In Situ Hybridization
Kaori Kobayashi and Hiroshi Kobayashi

Tumor Necrosis Factor-a Quantification and Expression by In Situ Hybridization
Tracey A. Ignatowski and Robert N. Spengler

Part III. Techniques for Molecular Biology

Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells
Ahmad Aljada, Husam Ghanim, and Paresh Dandona

Activation of Nuclear Factor-kB (NF-kB) in Mononuclear Cells (MNC)
Ahmad Aljada, Husam Ghanim, and PareshDandona

Assay of Intracellular Hydrogen Peroxide Generation in Activated Individual Neutrophils by Flow Cytometry
Yoshikazu Ito and David A. Lipschitz

DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)
Gieri Camenisch, Roland H. Wenger, and Max Gassmann

Analysis of Heat-Shock Transcription Factor and Element-Binding Activity
Yong J. Lee

Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation
Enbo Ma and Gabriel G. Haddad

An Expression System for a Transporter of Iron and Other Metals
Michael D. Garrick and Kevin G. Dolan

Analysis of Gene Expression Following Oxidative Stress
Dana R. Crawford, Toshihide Suzuki, Jan Sesay, and Kelvin J. A. Davies

Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis
Andrew Chow, Shahid Ahmed, Larrisa Chaplia, and Joseph Mattana

Thioredoxin and Redox Regulation of the Nuclear Receptor
Yuichi Makino, Kensaku Okamoto, and Hirotoshi Tanaka

Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA
Xin Gen Lei and Wen-Hsing Cheng

Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase
Kunio Yagi, Sadaaki Komura, and Nobuko Ohishi

Part IV. Techniques for In Vivo/Ex Vivo/In Vitro Applications

Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method
Takashi Sakurai and Susumu Terakawa

In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy
David M. Hall and Garry R. Buettner

Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Biopsies at 9.5 GHz
Jürgen Fuchs, Norbert Groth, and Thomas Herrling

In Vivo/In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy
Pei Tsai, Supatra Porasuphatana, Howard J. Halpern,


"...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement." - E-STREAMS
EAN: 9780896038516
ISBN: 0896038513
Untertitel: Ultrastructure and Molecular Biology Protocols. 'Methods in Molecular Biology'. 2002. Auflage. Sprache: Englisch.
Erscheinungsdatum: Juli 2002
Seitenanzahl: 356 Seiten
Format: gebunden
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