PCR in Bioanalysis

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Januar 1998



The invention of the polymerase chain reaction (PCR) eventually earned Kary B. Mullis half of the 1993 Nobel Prize for Chemistry (1-4). However, for several years, issues of quality control and reproducibility interfered with attempts at commercial or clinical application of PCR. More recently, persistent work and numerous methodological inno- tions and refinements have resulted in the establishment of PCR as a routine, sensitive, and specific detection method in hospital and agricultural labora- ries. This transformation of PCR from an experimental research technique to an established bioassay tool formed the impetus behind PCR in Bioanalysis. PCR has proven particularly valuable in clinical microbiology and the diagnosis of infectious diseases in humans and animals. This large and diverse group of applications is reviewed in Chapter 1 by Gorm Lisby. S- cific organisms now detectable by PCR include hepatitis C virus (protocols presented in Chapter 14), Mycobacterium tuberculosis (Chapter 18), Chla- dia and Trichomonas species (Chapter 20), Toxoplasma gondii (Chapter 17), Legionella species (Chapter 15), enterotoxigenic Escherichia coli (Chapters 9 and 10), HIV-1 subspecies (Chapter 8), bovine immunodeficiency-like virus (Chapter 6), rodent parvoviruses (Chapter 2), Ross River virus (Chapter 12), and porcine reproductive and respiratory syndrome virus (Chapter 7).


Application of Nucleic Acid Amplification in Clinical Microbiology
Gorm Lisby

Detection of Rodent Parvoviruses by PCR
David G. Besselsen

The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity
Darryl Shibata

Prenatal and Presymptomatic Diagnosis of Marfan Syndrome Using Fluorescence PCR and an Automated Sequencer
Mei Wang and Maurice Godfrey.

Measurement of TNF and iNOS mRNA Using cDNA-Equalized Reverse Transcriptase PCR
Jay K. Kolls and Jianming Xie

PCR Diagnosis of the Bovine Immunodeficiency-Like Virus
David L. Suarez and Cecelia A. Whetstone

PCR Analysis for the Identification of Porcine Reproductive and Respiratory Syndrome Virus in Boar Semen
Jane Christopher-Hennings and Eric A. Nelson

Quantitative and Discriminative Detection of Individual HIV-mRNA Subspecies by an RNase Mapping Assay
Catherine Mary, Hideo Akaoka, and Bernard Verrier

Detection of the Heat-Labile Toxin Coding Gene (LT-Gene) of Enterotoxigenic Escherichia coli
Séamus Fanning, Deirdre O'Meara, Lesley Cotter, John O'Mullane, and Bartley Cryan

Motif-Dependent Polymerase Chain Reaction (PCR): DNA Fingerprinting Entererotoxigenic Escherichia coli
Séamus Fanning, Deirdre O'Meara, Lesley Cotter, Paddy Greer, and Bartley Cryan

In Vitro Transcription/Translation Analysis for the Identification of Translation-Terminating Mutations
Michael C. Luce, Cameron G. Binnie, Lauren N. W. Kam-Morgan, and Matthew C. Cayouette

A Single-Tube Nested RT-PCR for the Detection of Ross River Virus
Loryn Sellner

Application of PCR to Transgenic Plants
Michael Wassenegger

Detection of Hepatitis C Virus RNA by Semiquantitative Reverse-Transcription PCR
Anderson S. Gaweco and David H. Van Thiel

Detection of Legionella Species in Bronchial Fluid by PCR

Quantitative Measurement of mRNA Expression by Competitive RT-PCR
Joe O'Connell, Triona Goode, and Fergus Shanahan

PCR Detection of Toxoplasma gondii in Human Fetal Tissues
Tamás Tóth, István Sziller, and Zoltán Papp

Rapid Diagnosis of Pulmonary Tuberculosis Using Roche AMPLICORTM Mycobacterium tuberculosis PCR Test
Richard F. D'Amato and Albert Miller

The Use of Flow Cytometry and RT-PCR in the Detection of Circulating PSA-Positive Cells in Prostate Cancer
Emma J. Fadlon and Freddie C. Hamdy

Detection of Chlamydia trachomatis and Trichomonas vaginalis in the Vaginal Introitus, Posterior Vagina, and Endocervix by Polymerase Chain Reaction
Jan Jeremias, Vera Tolbert, and Steven S. Witkin

Detection and Isolation of Differentially Expressed Genes by PCR
John M. Abraham

Detection of Transgene Integrants and Homologous Recombinants in Mice by Polymerase Chain Reaction
Kristin M. Abraham, Nancy S. Longo, and Judith A. Hewitt

Direct Analysis for Familial Adenomatous Polyposis Mutations
Steven M. Powell

PCR Fingerprinting for Detection of Deleted or Amplified Sequences in Human Cancer
Takashi Kohno and Jun Yokota

EAN: 9780896034976
ISBN: 0896034976
Untertitel: 1998. Auflage. Book. Sprache: Englisch.
Verlag: Humana Press
Erscheinungsdatum: Januar 1998
Seitenanzahl: 300 Seiten
Format: gebunden
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