Food-Borne Pathogens: Methods and Protocols

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Oktober 2005



A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


Part I. The Bacteria

Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources: PCR Analysis and Biological Activity
Rosabel Falcón, Tatiana d'Albuquerque e Castro, Maria das Graças de Luna, Angela Corrêa de Freitas-Almeida, and Tomomasa Yano

Detection and Purification of Bacillus cereus Enterotoxins
Toril Lindbäck and Per Einar Granum

Campylobacter: Isolation, Identification, and Preservation
Rachel Gorman and Catherine C. Adley

Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces
Miia Lindström, Mari Nevas, and Hannu Korkeala

Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex
Peter C. H. Feng and Steven R. Monday

PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis
Lewis M. Graves and Balasubramanian Swaminathan

Plesiomonas shigelloides: Detection by PCR
Ivan Ciznar, Carlos González-Rey, Karel Krovacek, and Anna Hostacka

Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies
Rachel Gorman and Catherine C. Adley

Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus
Moira M. Brett

Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples
Keya De, Ranjan K. Nandy, and G. Balakrish Nair

HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Jennifer Colford

Part II. The Viruses

Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR
Christian M. Beuret

Detection of Enteroviruses
Miguel-Angel Jiménez-Clavero, Victoria Ley, Nuria Gómez, and Juan-CarlosSáiz

Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification
Julie Jean and Ismaïl Fliss

Part III. The Parasites

Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis
Sandra M. O'Neill, Grace Mulcahy, and John P. Dalton

Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish
Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, and John E. Moore

Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.)
Giuseppe La Rosa, Stefano D'Amelio, and Edoardo Pozio

Part IV. Parallel Studies to the Analysis of Food-Borne Pathogens

Approaches to Developing Quantitative Risk Assessment Models
Enda J. Cummins

A Review of Surveillance Networks of Food-Borne Diseases
Camelia Molnar, Rita Wels, and Catherine C. Adley

EAN: 9781588294654
ISBN: 158829465X
Untertitel: Methods and Protocols. 'Methods in Biotechnology'. 2006. Auflage. Sprache: Englisch.
Erscheinungsdatum: Oktober 2005
Seitenanzahl: 268 Seiten
Format: gebunden
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