Yeast Gene Analysis

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Juli 2007



Focusing on Saccharomyces cerevisiae, this second edition covers the major genome-wide strategies such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches.


Preface Introduction to functional analysis in yeast Yeast genetics and strain construction Yeast transformation A guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting) Studying essential genes: generating and using promoter fusions and conditional alleles Yeast hybrid approaches Array-based yeast two-hybrid screening for protein-protein interactions Reporter genes and their uses in studying yeast gene expression Transcript analysis: a microarray approach GFP-based microscopic approaches for whole chromosome analysis in yeasts Immunological methods Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFP Identification, characterization, and phenotypic analysis of covalently linked cell wall Proteins Yeast protein microarrays Smart genetic screens High-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiae Chemical genomic tools for understanding gene function and drug action RNA gene analysis Analysis of gene function of mitochondria Yeast prions and their analysis in vivo Metabolic control in the eukaryotic cell, a Systems Biology perspective Phylogenetic footprinting Saccharomyces cerevisiae as a tool for human gene function discovery Bioinformatic prediction of yeast gene function Yeast genetic strain and plasmid collections Yeast gene analysis: the future
EAN: 9780123694782
ISBN: 0123694787
Untertitel: Sprache: Englisch.
Erscheinungsdatum: Juli 2007
Seitenanzahl: 700 Seiten
Format: gebunden
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