The Protein Protocols Handbook

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Oktober 2009



Since the publication of the bestselling second edition of John Walker's widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology(TM) series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armamentarium of techniques in the urgent search for rapid and robust results



1. Protein Determination by UV Absorption

A. Aitken and M.P. Learmonth

2. The Lowry Method for Protein Quantitation

J. H. Waterborg

3 The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

J.M. Walker

4 The Bradford Method for Protein Quantitation


5. Ultrafast Protein Determinations Using Microwave Enhancement

R. Akins and R. S. Tuan

6. The Nitric Acid Methods for Protein Estimation in Biological Samples

S.A. Boerner, Y.K. Lee, S.H. Kaufmann and K. Bible

7. Quantitation of Tryptophan in Proteins

A. Aitken and M.P. Learmonth

8. Kinetic Silver Staining of Proteins

D. D. Root and K. Wang

9. Quantitation of cellular proteins by flow cytometry

T. D. Friedrich , F.A. Ray, R.L. Smith and J. M. Lehman
10. Quantitation of cellular proteins by laser scanning cytometry

T.D. Friedrich, R.L.Smith and J.M. Lehman


11 Protein Solubility in 2D Electrophoresis: Basic principles and issues

12 Mouse and Human Tissues Sample Preparation for 2-D Electrophoresis

C. Zabel and J. Klose
13 Plant Protein sample preparation for 2DE

S. Carpentier , R.Swennen and B. Panis

14 Preparation of bacterial samples for 2-D PAGE.

B. Vandahl, G. Christiansen and S. Birkelund
15 Preparation of bodily fluids for 2-D PAGE
Sullivan, H.Brzeski, J. Ganesalingam, and M. Mayr
16 Immunoaffinity Depletion of high abundance plasma and serum proteins
L.A. Echan and D. Speicher

17 Preparation of Yeast samples for 2D PAGE

J. Norbeck

18 Membrane Protein Preparation Using Aqueous Polymer Two Phase Systems
J. Schindler and H-G Nothwang
19 Subcellular fractionation of small sample amounts

H.G Nothwang, I. Guillemin and J. Schindler
20. Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins

J.M. Walker

21 SDS Polyacrylamide Gel Electrophoresis of Proteins

J.M. Walker

22 Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins

J.M. Walker

23. SDS-Polyacrylamide Gel Electrophoresis of Peptides

R. C. Judd

24 Blue native gel electrophoresis (BN-PAGE)

Elke A. Dian, J. Rassow and Christian Motz

25 Separation of proteins by gel electrophoresis in the Tris-Taurine-HCl system

S.Luche, M. Chevallet C. Lelong and T. Rabilloud
26 Cetyltrimethylammonium Bromide Discontinuous Gel Electrophoresis of Proteins: M -Based Separation of Proteins with Retained Native Activity

R.E. Akins and R. S. Tuan

27 Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Basic Proteins

J. H. Waterborg

28 Acid-Urea-Triton Polyacrylamide Gel Electrophoresis of Histones

J. H. Waterborg

29 Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels

J. M. Walker

30 Serial immobilized pH gradient isoelectric focusing over pH 4-9

S.Poznanovic, W. Wozny, H. Zengerling, G.P. Schwall and M. A. Cahill
31. Radiolabelling of Eukaryotic Cells and Subsequent Preparation for 2-D <
M. Page and R. Thorpe

188 Analysis of IgG Fractions by Electrophoresis

M. Page and R. Thorpe

189 Purification of Immunoglobulin Y (IgY) from Chicken Eggs

C.R. Bird and R. Thorpe

190 Affinity Purification of Immunoglobulins Using PAM (Protein A Mimetic)
G. Fassina G. Palombo, A. Verdoliva and M. Ruvo

191 Detection of Serological Cross Reactions by Western Cross Blotting

P. Hemmerl, A. Hartl, J. Freund and J.Thalhamer
192 Enzymatic Digestion of Monoclonal Antibodies

S. Andrew

193 How to Make Bispecific Antibodies
R. French

194 Antigen Measurements Using ELISA

W. Jordan

195 Enhanced chemiluminescence immunoassay

R. Stott

196 Immunoprecipitation and Blotting

S. Thompson

197 Determination of Epitope by Mass Spectrometry

C.Hager-Braun and K. B. Tomer

198 Immunogen Preparation and Immunization Procedures for Rats and Mice

M. Page and R. Thorpe

199 Making Hybridomas

R. Burns

200 Growing hybridomas

G. Entrican, C. Jepson and D. Deane

201 Mouse Hybridomas as an Entryway to Monoclonal Antibody Design
and Production

E. Mechetner

202 Pitfalls

E. Mechetner

203 Recombinant antibody expression and purification
A. Knappik and R. Brundiers

204 Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding

M. Page and R. Thorpe

205 Screening Hybridoma Culture Supernatants Using ELISA

M. Page and R. Thorpe

206 Growth and Purification of Murine Monoclonal Antibodies

M. Page and R. Thorpe

207 Affinity purification techniques for monoclonal antibodies

A. Schwarz

208 A rapid method for generating large numbers of high-affinity monoclonal antibodies from a single mouse

N.T. Man and G.E. Morris
EAN: 9781588298805
ISBN: 1588298809
Untertitel: 'Springer Protocols Handbooks'. Sprache: Englisch.
Erscheinungsdatum: Oktober 2009
Seitenanzahl: 1991 Seiten
Format: gebunden
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